Data File  : C:\Chem32\1\Data\labstudenti\BCY5 2018-11-2016-33-44.D
Acq. Method: DidatticaLCMS_finale.M


The Acq. Method's Instrument Parameters for the Run were : 


=====================================================================
                              Quat. Pump
=====================================================================

Quat. Pump (G1311B)
===================

Low Pressure Limit:                                 0.00 bar
High Pressure Limit:                              390.00 bar
Flow:                                           0.250 mL/min
Maximum Flow Gradient:                         1.000 mL/min²
Primary Channel:                                           A

Stop Time                     
Stoptime Mode:                                      Time set
Stoptime:                                          55.00 min

Post Time                     
Posttime Mode:                                      Time set
Posttime:                                          10.00 min

Compress                      
Compressibility Mode:              Compressibility Value Set
Compressibility:                                46 10e-6/bar

Stroke                        
Automatic Stroke Calculation:                             No
Stroke:                                                20 µL
Solvent Composition
   Channel   Name 1   Used   Percent
                                   %
------------------------------------
         A             Yes       0.0
         B              No          
         C      H2O    Yes      97.0
         D      ACN    Yes       3.0



Timetable
   Time   A   B    C    D   Flow   Pressure
    min   %   %    %    % mL/min        bar
-------------------------------------------
  20.00 0.0 0.0 40.0 60.0  0.250     390.00
  50.00 0.0 0.0 20.0 80.0  0.250     390.00
  55.00 0.0 0.0 97.0  3.0  0.250     390.00






Instrument Curves
Store Pressure:                                           Yes
Store Flow:                                               Yes
Store Solvent Ratio A:                                    Yes
Store Solvent Ratio B:                                    Yes
Store Solvent Ratio C:                                    Yes
Store Solvent Ratio D:                                    Yes
Store Direction of Piston A:                              Yes






=====================================================================
                               Sampler
=====================================================================

Sampler (G1329B)
================


Stop Time                     
Stoptime Mode:                              As pump/No limit

Post Time                     
Posttime Mode:                                           Off

Auxiliary                     
Draw Speed:                                       500 µL/min
Eject Speed:                                      600 µL/min
Draw Position Offset:                                 0.0 mm

Injection                     
Injection Mode:                   Injection with needle wash
Injection Volume:                                   20.00 µL

Needle Wash                   
Needle Wash Location:                              Wash Vial
Wash Location:                                       Vial 91

High throughput               

Overlapped Injection          
Enable Overlapped Injection:                              No

Timetable                     








=====================================================================
                             Column Comp.
=====================================================================

Column Comp. (G1316A)
=====================

Valve Position:                     Position 2 (Port 1 -> 6)

Left Temperature Control      
Temperature Control Mode:                    Temperature Set
Temperature:                                        25.00 °C

Enable Analysis Left Temperature
Enable Analysis Left Temperature On:                     Yes
Enable Analysis Left Temperature Value:               0.8 °C

Right Temperature Control     
Right temperature Control Mode:              Temperature Set
Right temperature:                                  25.00 °C

Enable Analysis Right Temperature
Enable Analysis Right Temperature On:                    Yes
Enable Analysis Right Temperature Value:              0.8 °C

Stop Time                     
Stoptime Mode:                              As pump/injector

Post Time                     
Posttime Mode:                                           Off

Timetable                     




Instrument Curves
Store Left Temperature:                                    No
Store Right Temperature:                                   No






=====================================================================
                                 DAD
=====================================================================

DAD (G1315C)
============

Peakwidth:        > 0.013 min (0.25 s response time) (20 Hz)
Slit:                                                   4 nm
UV Lamp Required:                                        Yes
Vis Lamp Required:                                        No

Analog Output 1               
Analog 1 Zero Offset:                                    5 %
Analog 1 Attenuation:                               1000 mAU

Analog Output 2               
Analog 2 Zero Offset:                                    5 %
Analog 2 Attenuation:                               1000 mAU

Signals                       
Signal table
   Acquire   Signal   Wavelength   Bandwidth   Use Ref.
                              nm          nm           
-------------------------------------------------------
       Yes Signal A          220           8        Yes
       Yes Signal B          280           8        Yes
        No Signal C                                    
        No Signal D                                    
        No Signal E                                    
        No Signal F                                    
        No Signal G                                    
        No Signal H                                    


   Ref Wavel.   Ref Bandw.
           nm           nm
--------------------------
          360          100
          360          100
                          
                          
                          
                          
                          
                          




Prepare Mode                  
Margin for negative Absorbance:                      100 mAU

Autobalance                   
Autobalance Prerun:                                      Yes
Autobalance Postrun:                                      No

Spectrum                      
Spectrum Range WL from:                               190 nm
Spectrum Range WL to:                                 400 nm
Spectrum Step:                                        2.0 nm
Spectrum Store:                                  All in Peak
Spectrum Threshold:                                 10.0 mAU

Stoptime                      
Stoptime Mode:                              As pump/injector

Posttime                      
Posttime Mode:                                           Off

Timetable                     




Instrument Curves
Store Board Temperature:                                   No
Store Optical Unit Temperature:                            No
Store UV Lamp Anode Voltage:                               No





=====================================================================
                       Mass Spectrometer Detector
=====================================================================


General Information
------- -----------

Use MSD                  : Enabled
Tune File                : atunes.tun
StopTime                 : No Limit
Time Filter              : Enabled
Data Storage             : Condensed
Peakwidth                : 0.10 min
Fast Scan                : Disabled
Fast Scan Data Reconstruction: Disabled
Polarity Switch Delay    : 300 ms
Ionization Switch Delay  : 50 ms


Signals
-------

[Signal 1]

Ionization Mode          : API-ES
Polarity                 : Positive
Fragmentor Ramp          : Disabled
Percent Cycle Time       : 50.00 %

Scan Parameters

   Time |      Mass Range    |Frag- | Gain|Thres-| Step-
  (min) |    Low   |  High   |mentor| EMV | hold | size
 -------|----------|---------|------|-----|------|-------
    2.00    100.00    800.00   150    1.0     150  0.10


[Signal 2]

Ionization Mode          : API-ES
Polarity                 : Negative
Fragmentor Ramp          : Disabled
Percent Cycle Time       : 50.00 %

Scan Parameters

   Time |      Mass Range    |Frag- | Gain|Thres-| Step-
  (min) |    Low   |  High   |mentor| EMV | hold | size
 -------|----------|---------|------|-----|------|-------
    2.00    100.00    800.00   150    1.0     150  0.10


[Signal 3]

Not Active


[Signal 4]

Not Active



Spray Chamber
----- -------

[MSZones]

Gas Temp                 : 350 C                  maximum 350 C
DryingGas                : 12.0 l/min             maximum 13.0 l/min
Neb Pres                 : 35 psig                maximum 60 psig
Quad Temp                : 0 C                    maximum 0 C

VCap (Positive)          : 3000 V
VCap (Negative)          : 3000 V

[Time Table]

Time Table is empty.



                      END OF MS ACQUISITION PARAMETERS

=====================================================================
                              FIA Series
=====================================================================

FIA Series in this Method   :      Disabled

Time Setting
    Time between Injections   :     0.19 min
    Injection Loop Flush Time :     0.17 min




