Section outline

  • 2.2. Thermal forces, diffusion, and chemical forces

    • Chemical forces; amino acids protein; types of forces;

    • Ligands : how they work and examples ( rhodopsin)

    • Chemical equilibria and the effect of force on chemical equilibria (ex hair

    • Rate theory of chemical reactions and the effect of force on the chemical rate constant ex myosin)

    • Examples of single molecule force spectroscopy experiments

    1. How to determine the dissociation rate of a ligand receptor bond using force

    (model and exp Adhesion through Single Peptide Aptamers

    2. Titin unfolding AFM vs OT

    3. OT configurations for force spectroscopy single molecule experiments examples

    4. The complex folding network of single calmodulin molecules using OT single molecule force spectroscopy