Section outline

    • Expression of recombinant proteins File PDF

      Expression of recombinant proteins for structural studies. Design of the protein construct. Selection of a suitable expression system. Main molecular biology techniques used in structural biology.

    • ExPASy URL

      Bioinformatics resource operated by the SIB Swiss Institute of Bioinformatics. It includes various bioinformatic tools, among which databases and online software for structural biology, medicinal chemistry, genomics, proteomics, and systems biology.

    • UniProt URL

      This protein sequence database includes annotations about localization, function, structure, post-translational modifications, etc. The website also contains links to bioinformatics tools for sequence alignment (Align) and primary sequence homology searches (Blast).

    • Green Fluorescent Protein (GFP) e suo cromoforo File AVI
    • Purification of recombinant proteins for structural studies File PDF

      Purification techniques. Methods to increase the stability, conformational homogeneity, and crystallizability of a protein. Evaluation of the quality and purity of the sample. Analysis of the conformational stability. Analysis of the oligomeric state of the protein.