588SM - Biofisica Sperimentale 2022
Schema della sezione
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Introduzione ai biopolimeri e la loro struttura
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Lezione1 File PDF
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Introduzione ai biopolimeri 2-Le proteine
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Lezione 2 File PDF
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Cristallografia a raggi X di macromolecole
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Lezione 3 File PDF
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Cryo Electron Microscopy in biofisica
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Lezione 4 File PDF
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Introduzione Atomic Force Microscopy
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Introduction, summary, references
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Lecture 1 File PDF
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2. Physical principles
2.1. Forces at molecular and cell level
• Physical forces and their magnitudes at the single molecule level
• Modeling complex mechanical devices as protein machines by using three elements
case study: Mass, Stiffness and Damping of Proteins
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Lecture 2 File PDF
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2.2. Thermal forces, diffusion, and chemical forces
• Boltzmann Distribution Law and the Principle of Equipartition of Energy
• Diffusion equation, Einstein relation, Stokes law
• Autocorrelation function and Power Spectrum - applications
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Lecture 3 File PDF
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2.2. Thermal forces, diffusion and chemical forces
• Chemical forces; amino acids - protein; types of forces;
• Ligands how they work and examples
• Chemical equilibria and the effect of force on chemical equilibria
• Rate theory of chemical reactions and the effect of force on the chemical rate constant
• Examples of single molecule force spectroscopy experiments unbinding, unfolding
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Lecture 4 File PDF
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Recommended papers related to the lectures presented in class.
One paper, selected by prof and communicated previously (2 days before exam), will be presented and discussed in detail by student at exam.
Power point presentation, max 15 min.
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Optical trapping and manipulation principlesExamples of OT applications in biophysics
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Lecture 5 File PDF
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