588SM - Biofisica Sperimentale 2022

Section outline

• Select section General

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  General

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  • Select activity Annunci

    

    Annunci Forum
• Select section Lezione 1

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  Lezione 1

  Introduzione ai biopolimeri e la loro struttura
  

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    Lezione1 File PDF
• Select section Lezione 2

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  Lezione 2

  Introduzione ai biopolimeri 2-Le proteine
  

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• Select section Lezione 3

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  Lezione 3

  Cristallografia a raggi X di macromolecole
  

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• Select section Lezione 4

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  Lezione 4

  Cryo Electron Microscopy in biofisica
  

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• Select section Testo riferimento forze intermolecolari

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  Testo riferimento forze intermolecolari

  • Select activity Israelachvili

    

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• Select section Lezione 5

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  Lezione 5

  Introduzione Atomic Force Microscopy
  

  • Select activity Lezione 5 - AFM1

    

    Lezione 5 - AFM1 File PDF
• Select section Lezioni 6-7 Membrane Biophysics

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  Lezioni 6-7 Membrane Biophysics

  • Select activity Membrane Biophysics 1

    

    Membrane Biophysics 1 File PDF
• Select section Lezione 8_AFM imaging membranes

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  Lezione 8_AFM imaging membranes

  • Select activity AFM Imaging membranes

    

    AFM Imaging membranes File PDF
• Select section Lezione 9_Force Spectroscopy

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  Lezione 9_Force Spectroscopy
• Select section Lezione 10_Membrane electrical model

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  Lezione 10_Membrane electrical model
• Select section Ptotein-protein interactions

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  Ptotein-protein interactions

  • Select activity Protein-protein interactions

    

    Protein-protein interactions File PDF
• Select section Cell Mechanics L1

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  Cell Mechanics L1

  Introduction, summary, references

  • Select activity Lecture 1

    

    Lecture 1 File PDF
  • Select activity Howard - Book- Ch_2

    

    Howard - Book- Ch_2 File PDF
  • Select activity Howard_Book_Ch_3

    

    Howard_Book_Ch_3 File PDF
  • Select activity Howard_Book_Ch_4

    

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  • Select activity Howard_Book_Ch_5

    

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• Select section Cell Mechanics L 2-3

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  Cell Mechanics L 2-3

  2. Physical principles

  2.1. Forces at molecular and cell level

  • Physical forces and their magnitudes at the single molecule level

  • Modeling complex mechanical devices as protein machines by using three elements

  case study: Mass, Stiffness and Damping of Proteins

  • Select activity Lecture 2

    

    Lecture 2 File PDF
• Select section Cell Mechanics L 4-5

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  Cell Mechanics L 4-5

  2.2. Thermal forces, diffusion, and chemical forces

  • Boltzmann Distribution Law and the Principle of Equipartition of Energy

  • Diffusion equation, Einstein relation, Stokes law

  • Autocorrelation function and Power Spectrum - applications

  
  

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    Lecture 3 File PDF
• Select section Cell Mechanics L 6-7-8

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  Cell Mechanics L 6-7-8

  2.2. Thermal forces, diffusion and chemical forces

  • Chemical forces; amino acids - protein; types of forces;

  • Ligands how they work and examples

  • Chemical equilibria and the effect of force on chemical equilibria

  • Rate theory of chemical reactions and the effect of force on the chemical rate constant

  • Examples of single molecule force spectroscopy experiments unbinding, unfolding 

  • Select activity Lecture 4

    

    Lecture 4 File PDF
• Select section Cell Mechanics Papers References

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  Cell Mechanics Papers References

  Recommended papers related to the lectures presented in class. 

  One paper, selected by prof and communicated previously (2 days before exam), will be presented and discussed in detail by student at exam.

  Power point presentation, max 15 min.  

  • Select activity 1997_Science_Bustamante

    

    1997_Science_Bustamante File PDF
  • Select activity 1999_PNAS_Fernandez

    

    1999_PNAS_Fernandez File PDF
  • Select activity 1995_PNAS_Howard

    

    1995_PNAS_Howard File PDF
  • Select activity 2005_Nature_Block

    

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  • Select activity 2011_J_Phys_Chem_Lang

    

    2011_J_Phys_Chem_Lang File PDF
  • Select activity 2011_Science_Stiegler

    

    2011_Science_Stiegler File PDF
  • Select activity 2012_POne_Navaz

    

    2012_POne_Navaz File PDF
  • Select activity 2015_ELife_Patapoutian_Piezo1 RBC mechanical activation

    

    2015_ELife_Patapoutian_Piezo1 RBC mechanical activation File PDF
• Select section Cell Mechanics L 9

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  Cell Mechanics L 9

  Optical trapping and manipulation principles

  
  

  Examples of OT applications in biophysics

  
  

  • Select activity Lecture 5

    

    Lecture 5 File PDF
  • Select activity Optical Tweezers - Review- BookChapter

    

    Optical Tweezers - Review- BookChapter File PDF