588SM - Biofisica Sperimentale 2023
Section outline
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- the cell as the basic unit of life, components and structure of a cell
- the importance of cell mechanics and mechanotransduction
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2.1. Forces at molecular and cellular levels
- Physical forces types and their magnitude at the single molecule level, with examples
- Modeling a complex mechanical device as a protein machine by using elementary mechanical elements
- Mass, stiffness and damping in proteins and cells
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2.2. Thermal forces, diffusion, and chemical forces
• Boltzmann Distribution Law and the Principle of Equipartition of Energy
• Diffusion equation - Einstein relation
• Autocorrelation function and Power Spectrum
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2.2. Thermal forces, diffusion, and chemical forces
• Chemical forces; amino acids protein; types of forces;
• Ligands : how they work and examples ( rhodopsin)
• Chemical equilibria and the effect of force on chemical equilibria (ex hair
• Rate theory of chemical reactions and the effect of force on the chemical rate constant ex myosin)
• Examples of single molecule force spectroscopy experiments
1. How to determine the dissociation rate of a ligand receptor bond using force
(model and exp Adhesion through Single Peptide Aptamers
2. Titin unfolding AFM vs OT
3. OT configurations for force spectroscopy single molecule experiments examples
4. The complex folding network of single calmodulin molecules using OT single molecule force spectroscopy
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Optical trapping and manipulation: principlesExamples of OT applications in biophysics:particles manipulation and force spectroscopy single cell
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Motor proteins (types, working principles)
To study optional, not requested for Exam
- Motor proteins classification
- Actin and microtubule based motor proteins.
- Force generation by Motor Proteins
- Muscle contraction
- Experiment example: a myosin II synthetic nanomachine
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