588SM - Biofisica Sperimentale 2021

Section outline

• Select section General

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  General

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  • Select activity Annunci

    

    Annunci Forum
  • Select activity Lezione 10

    

    Lezione 10 Lesson
• Select section Lezione 1 Introduction

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  Lezione 1 Introduction

  Cell Mechanics / Meccanobiologia Cellulare

  1. Introduction

  •The cell as the basic unit of life, components and structure of a cell

  •The importance of cell mechanics and mechanotransduction

  
  

  • Select activity Lezione 1 Cojoc

    

    Lezione 1 Cojoc File PDF
• Select section Lezione 2 Physical Principles

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  Lezione 2 Physical Principles

  Cell Mechanics / Meccanobiologia Cellulare
  

  2. Physical Principles

  2.1.  Forces at molecular and cell level
  

  •Physical forces and their magnitudes at the single-molecule level

  •Modeling complex mechanical devices as protein machines by using three elements:   

  Case study: Mass, Stiffness and Damping of Proteins

  
  

  • Select activity Lezione 2 Physical Principles

    

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  • Select activity Book_Howard_Ch_2

    

    Book_Howard_Ch_2 File PDF
  • Select activity Book_Howard_Ch_3

    

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• Select section Lezione 3 Physical Principles

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  Lezione 3 Physical Principles

  Cell Mechanics / Meccanobiologia cellulare

  2. Physical Principles

  2.2.  Thermal forces, diffusion, and chemical forces

  •Boltzmann’s law and the  Principle of Equipartition of Energy

  •Diffusion equation - Einstein relation – Stokes law

  •Autocorrelation function and Power Spectrum

  
  

  • Select activity Lezione 3 Physical Principles

    

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  • Select activity Book_Howard_Ch_4

    

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• Select section Lezione 4 Physical Principles

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  Lezione 4 Physical Principles

  Cell Mechanics / Meccanobiologia cellulare

  2. Physical Principles

  2.2.  Thermal forces, diffusion, and chemical forces

  • The effect of force o the equilibria and rate of chemical reactions

  • Examples of the use of mechanical force in single molecule experiments - unbinding ligand-receptor, unfolding protein
  

  
  

  • Select activity Book_Howard_Ch_5

    

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  • Select activity Lezione 4 - Physical principles

    

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• Select section Lezione 5 - Optical Tweezers

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  Lezione 5 - Optical Tweezers

  -Optical trapping and manipulation principles: an homage to Arthur Ashkin 

   - Examples of OT applications in biophysics
  

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• Select section Lezione 6 - Motor proteins

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  Lezione 6 - Motor proteins

  3.1. Motor proteins

  3.1.1.Motor proteins classification

  3.1.2. Actin- and microtubule- based motor proteins.

  3.1.3. Force generation by Motor Proteins

  3.1.4. Motor proteins and cellular functions

  3.1.5. Muscle contraction

  
  

  • Select activity Lezione 6 - Motor proteins

    

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• Select section Lezione 7 - Cytoskelteon

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  Lezione 7 - Cytoskelteon

  3.2. Cytoskeleton structure and Force generation by the cytoskeleton.

  • Cytoskeleton filaments: types, structure, localization and function

  • Cytoskeleton filaments polymerization and depolymerization.

  • Forces generated by polymerization / depolymerization

  • Cell motility induced by cytoskeleton filaments, examples.

  
  

  • Select activity Lezione 7 - Cytoskeleton

    

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• Select section Lezione 8 - Mechanosignaling and experimental techniques

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  Lezione 8 - Mechanosignaling and experimental techniques

  4. Experimental techniques to study cell mechanics and mechanotransduction
  

  •  What is mechanosignaling and what types of forces do cells encounter ?
  •  Examples of mechanotransduction and experimental techniques: magnetic tweezers, acoustic tweezers, pipette aspiration, traction force microscopy, optical stretcher, optical tweezers

  
  

  • Select activity Lezione 8 - Mechanosignaling and experimental techniques

    

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• Select section Lezione 9 - Optical Nanoscopy

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  Lezione 9 - Optical Nanoscopy

  Super resolution optical microscopy or Optical Nanoscopy

  • STED - Stimulated Emission Depletion 
  • PALM - Photo Activated Localization Microscopy

  • Select activity Modifica

    

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  • Select activity Lezione 9 - Optical Nanoscopy

    

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• Select section Lezione 10

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  Lezione 10

  • Select activity Macromolecules

    

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  • Select activity Methods in Molecular Biophysics- Serdyuk,Zaccai, Zaccai

    

    Methods in Molecular Biophysics- Serdyuk,Zaccai, Zaccai File PDF
  • Select activity Intermolecular and surface forces-Israelachvili

    

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• Select section Lezione 11

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  Lezione 11

  • Select activity Molecular Crystallography

    

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• Select section Lezione 12

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  Lezione 12

  • Select activity Electron Microscopy

    

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• Select section Lezione 13

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  Lezione 13

  • Select activity Atomic Force Microscopy_1

    

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• Select section Lezione 14

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  Lezione 14

  • Select activity AFM Imaging_membranes

    

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• Select section Lezione 15

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  Lezione 15

  • Select activity AFM_Imaging_Single Molecule

    

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• Select section Lezione 16

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  Lezione 16

  • Select activity AFM_ForceSpectroscopy

    

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• Select section Lezione 17

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  Lezione 17

  • Select activity Membranes biopgysics

    

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• Select section Lezione 18

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  Lezione 18

  • Select activity Cell-Cell communication and action potential

    

    Cell-Cell communication and action potential File PDF
• Select section Lezione 19

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  Lezione 19

  • Select activity Protein binding

    

    Protein binding File PDF
• Select section Lezione 20

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  Lezione 20