FA02092FA2022: VIDEO Purificazione e analisi di proteine | Moodle@Units

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Biochimica applicata (Purification and Analysis of Proteins)_CTF_23_24

An Introduction to the Centrifuge

Separation of Mixtures via Precipitation

Dialysis: Diffusion Based Separation

Principles Of Column Chromatography

Types Of Column Chromatography

Column Chromatography

Ion-Exchange Chromatography

Chromatography-based Biomolecule Purification Methods

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

High-Performance Liquid Chromatography (HPLC)

Anion-Exchange Chromatography-Based Protein Purification: A Separation Technique to Isolate a Protein of Interest From Dialyzed Bacterial Lysate Based on Net Charge

Anion-Exchange Chromatography-Based Protein Purification: A Separation Technique to Isolate a Protein of Interest From Dialyzed Bacterial Lysate Based on Net Charge

Cation Exchange Chromatography: A Technique to Isolate Target Recombinant Protein from Insect Cell Lysate Based on Net Surface Charge

Cation Exchange Chromatography: A Technique to Isolate Target Recombinant Protein From Insect Cell Lysate Based on Net Surface Charge

Two-dimensional Gel Electrophoresis

Separating Protein with SDS-PAGE

Two-Dimensional Gel Electrophoresis

Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads

MALDI-TOF Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Introduction to Mass Spectrometry

Tandem Mass Spectrometry

MALDI-TOF Mass Spectrometry

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids