Part 1. Lectures 4-5-6 OM Fluorescence Microscopy and Non Linear Optical Microscopy
Schema della sezione
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Outline
1. Epifluorescence basics;
- confocal; two photons
2. Super-resolution techniques:
- STtimulated Emission Depletion STED
- PhotoActivated Localization Microscopy PALM
- MINFLUX 3D nanoscopy
3. Forster Resonance Energy Transfer FRET microscopy
4. Photobleaching-based Techniques for Assessing Cellular Dynamicsst:
- Fluorescence recovery after photobleaching FRAP,
- Fluorescence Loss in Photobleaching FLIP
- Fluorescence Localization After Photobleaching FLAP,
- Photo Activation PA
5. Non Linear Optical microscopy
- : Two Photon Excitation Fluorescence (TPEF),
- Second Harmonic Generation (SGH),
- Coherent Raman Scattering (CRS)