607SM - TECNICHE AVANZATE DI INDAGINE MICROSCOPICA 2022

Section outline

• Select section General

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  General

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  • Select activity Annunci

    

    Annunci Forum
• Select section Part 1. Lezione 1 - Introduction

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  Part 1. Lezione 1 - Introduction

  • Select activity Part 1. Lecture 1 - Introduction

    

    Part 1. Lecture 1 - Introduction File PDF
• Select section Part 1. Lectures 2-3 Optical microscopy - unstained samples

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  Part 1. Lectures 2-3 Optical microscopy - unstained samples

  Outline

  1. Why optical microscopy? 

  2. Image formation; magnification and diffracted limited resolution

  3. Optical aberrations resolution and image quality

  4. Digital camera image acquisition (formats, properties, SNR) 

  5. Phase imaging techniques 

  • Phase contrast and differential interference contrast  (DIC); 
  • Quantitative phase imaging: digital holographic microscopy 

  6. Other techniques to image unstained samples

  • Dark field microscopy 
  • Polarization microscopy 

  • Select activity Part 1. Lectures 2-3 Optical microscopy - unstained samples

    

    Part 1. Lectures 2-3 Optical microscopy - unstained samples File PDF
  • Select activity Supplementary Material (SM) - Geometrical Optics

    

    Supplementary Material (SM) - Geometrical Optics File PDF
• Select section Part 1. Lectures 4-5-6 OM Fluorescence Microscopy and Non Linear Optical Microscopy

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  Part 1. Lectures 4-5-6 OM Fluorescence Microscopy and Non Linear Optical Microscopy

  Outline

  1. Epifluorescence basics; 
  

  • confocal; two photons

  2. Super-resolution techniques: 

  • STtimulated Emission Depletion STED 
  • PhotoActivated Localization Microscopy PALM
  •  MINFLUX 3D nanoscopy

  3. Forster Resonance Energy Transfer FRET microscopy

  4. Photobleaching-based Techniques for Assessing Cellular Dynamicsst: 

  • Fluorescence recovery after photobleaching FRAP, 
  • Fluorescence Loss in Photobleaching FLIP
  • Fluorescence Localization After Photobleaching FLAP, 
  • Photo Activation PA

  5. Non Linear Optical microscopy
  

  • : Two Photon Excitation Fluorescence (TPEF),
  •   Second Harmonic Generation  (SGH),
  •   Coherent Raman Scattering (CRS)

  
  

  
  

  • Select activity 2012 Review - Advanced Fluorescence Microscopy Techniques

    

    2012 Review - Advanced Fluorescence Microscopy Techniques File PDF
  • Select activity 2015 Research paper STED neurons

    

    2015 Research paper STED neurons File PDF
  • Select activity 2012 Research paper RESOLFT neurons

    

    2012 Research paper RESOLFT neurons File PDF
  • Select activity 2017 Research paper MINFLUX

    

    2017 Research paper MINFLUX File PDF
  • Select activity 2020 REVIEW Non Linear Optical microscopy

    

    2020 REVIEW Non Linear Optical microscopy File PDF
  • Select activity 2019 Intro to NLO

    

    2019 Intro to NLO File PDF
  • Select activity Lecture 4-5-6 Fluorescence - Super Resolution . Non Linear Optical Microscopy

    

    Lecture 4-5-6 Fluorescence - Super Resolution . Non Linear Optical Microscopy File PDF
• Select section Parts 1. Lecture 7 Electron Microscopy

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  Parts 1. Lecture 7 Electron Microscopy

  Outline

  1. What is Electron Microscopy and why it is used

  2. Interaction electron beam - matter and contrast mechanism for image formation

  3. Types of EM:

  • Transmission Electron Microscopy TEM
  • Scanning Electron Microscopy SEM
  • EM application to biological samples

  4. Cryo- EM - why and how for biological samples

  5. Focused Ion Beam - what and why - basics  
  

  • Select activity Lecture 7 Electron Microscopy

    

    Lecture 7 Electron Microscopy File PDF
• Select section Part 1. Lectures 8-9 X-ray Microscopy

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  Part 1. Lectures 8-9 X-ray Microscopy

  Outline

  
  

  1.Why X-Ray microscopy ?

  
  

  2.How is the image formed in X-ray microscopy ?

  
  

  3.X-ray microscope components and techniques

  
  

  4.Radiation damage

  
  

  5.X-ray microscopy with Syncrotron light

  
  

  6.Imaging cell morphology and physiology using X-rays (review to read)

  
  

  7.X-ray  diffraction imaging and Free Electron Laser (FEL)

  
  

  • Select activity Lecture 8-9 X-Ray Microscopy

    

    Lecture 8-9 X-Ray Microscopy File PDF
• Select section Part 1. Lecture 10 Atomic Force Microscopy

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  Part 1. Lecture 10 Atomic Force Microscopy

  Outline

  
  

  1. Why Atomic Force Microscopy ?

  
  

  2. Scanning Tunneling Microscopy STM and AFM – working principle

  
  

  3. Force vs tip-substrate distance

  
  

  4. AFM - operation modes:

     • imaging or force spectroscopy

     • static and dynamic

  
  

  5. Examples of AFM imaging in biology / life sciences 

  
  

  • Select activity Lecture 10 Atomic Force Microscopy

    

    Lecture 10 Atomic Force Microscopy File PDF
• Select section Part 1. Lectures 11-12 Micro and nano particle manipulation and force spectroscopy

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  Part 1. Lectures 11-12 Micro and nano particle manipulation and force spectroscopy

  Outline

  
  

  1.Why Optical, Acoustic, Magnetic Tweezers ?

  
  

  2.Optical Tweezers (OT) working principle

  
  

  3.OT particle manipulation in biological applications

  
  

  4.Acoustic  and Magnetic Tweezers working principle

  
  

  5.AT and MT in biological applications

  
  

  6.Single molecule biology - force spectroscopy

  
  

  • Select activity Lectures 11-12. Micro and nano particle manipulation and force spectroscopy

    

    Lectures 11-12. Micro and nano particle manipulation and force spectroscopy File PDF
• Select section Topic 8

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  Topic 8
• Select section Topic 9

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  Topic 9
• Select section Topic 10

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  Topic 10