675SM - MICROSCOPIA OTTICA IN BIOLOGIA CELLULARE 2023
Schema della sezione
-
-
Lesson 1 File PDF1.Lesson 1 - How a microscope works
1.1. Image formation
1.2. Magnification vs resolution
1.3. Numerical aperture and working distance
1.4. Objectives
1.5. Point-spread function and Airy disk
1.6. Optical abberations
-
-
-
Lesson 2 File PDF
2. Lesson2 + Lab – Contrasting techniques
2.1. Brightfield
2.2. Darkfield
2.3. Phase Contrast
2.4. Polarization Contrast
2.5. Differential Interference Contrast (DIC)
-
-
-
Lesson 3 File PDF
3. Lesson 3 - Flourescence microscopy
3.1. Flourescence principle
3.2. Absorption and Emission spectra - Stoke’s shift
3.3. The flourescence microscope –
light sources, filter, dicroic mirror
3.4. Fluorophores
3.5. Staining with flourophores
3.6. Problems with flourescence imaging
3.7. Multichannel imaging
-
-
-
Lesson 4 File PDF
4. Lesson 4 – Confocal, super-resolution and 2-photon microscopy
4.1. TIRF microscopy
4.2. Confocal microscopy
4.3. 2-photon microscopy
4.4. Superresolution microscopy
4.4.1. SIM microscopy
4.4.2. STED microscopy
4.4.3. PALM microscopy
4.4.4. STORM microscopy
4.5. FRET microscopy
4.6. FRAP microscopy
-